Praktikum Practical course C elegans as an experimental model

Course content: Participants in -C. elegans as an experimental model” obtain basic knowledge of C. elegans biology and are introduced to procedures such as C. elegans handling, microscopy (stereo-, DIC- and fluorescence), gene discovery through mutant screening, various phenotypic analyses and genetic analyses (genetic crosses, classical and SNP mapping etc). During the course, each student will also obtain an unknown mutant, which the student needs to analyze phenotypically and molecularly to identify the gene that is affected in the particular mutant. Emphasis is placed on hands-on practice with the techniques mentioned above and interpretation of the data obtained. The lab entails 6 SWS, and requires a detailed lab report according to excellent scientific practice. Qualification goals: Students can apply theoretical and practical knowledge to approach biological questions in independent work. Students obtain skills for future lab work with C. elegans and other model organisms, in particular in preparation for their master’s thesis. Students are well trained in good general lab practice, including standard safety procedures, precise handling of chemicals and instruments, conscientious documentation of lab procedures, and obtain skills in specialized techniques such as C. elegans handling, microscopy (stereo-, DIC- and fluorescence), gene discovery through mutant screening, various phenotypic analyses and genetic analyses (genetic crosses, classical and SNP mapping etc). Students practice critical evaluation and interpretation of data as a basis for careful and relevant conclusions. In working in small lab groups (2 students), social skills (teamwork, cooperation, fair play, work delegation, mutual respect), communication skills (rapport with instructors and fellow students, presentations, written lab reports), as well as organizational skills (efficient planning, documentation) are refined. Students learn excellent scientific methods in written data presentation, including well-founded introduction to the topic, documentation, interpretation and discussion of the results. These skills are particularly aimed at preparing students for theses writing and scientific publications. Lambie, Eric , PD Dr. LMU München WiSe 1617 Univ.Prof.Dr. Conradt Barbara