Praktikum Practical course DNA Repair

Course Content: In the context of a general overview on cellular response mechanisms to DNA damage, participants are introduced to several current questions such as genetic and miRNA network controlling the DNA damage response mechanisms, or influence of specific inhibitors. Participants learn handling and cultivation of mammalian cells, treatment with inhibitors, knock-down by RNA interference, indirect immunolabeling of fixed cells, fluorescence light microscopy and image analysis by imageJ, protein isolation and Western blot-based protein analysis, RNA isolation and RT-PCR, FACS analysis of cell cycle distribution and apoptosis. Emphasis is placed on hands-on practice in small groups (max. 8 participants), planning of experiments with appropriate controls, and interpretation of data. For presentation of results, the participants produce scientific posters. The lab entails 3 SWS. Qualification goals: The students are proficient in basic knowledge on DNA damage response mechanisms. Students can apply theoretical and practical knowledge to approach biological questions in independent work. Students can apply introduced methods in future lab work, in particular in preparation for their master’s thesis. Students learn excellent scientific method in data presentation, especially in preparation of posters. These skills are particularly aimed at preparing students for participation in scientific conferences. Students are equipped for scientific research in this topic and can discuss this in a broad context. Mörtl, Simone , Dr. LMU München WiSe 1617 PD Dr. Friedl Anna