Praktikum Lecture and Practical course Prokaryote eukaryote interactions

Course Content: The practical course contains experiments on the topics microbial identification and localization using molecular phylogenetic tools, molecular tagging with green-fluorescent protein, immunochemical methods (like enzyme-linked immunostaining assay) and advanced microscopic techniques (like confocal laser scanning microscopy). The course teaches not only to understand and perform in detail complex protocols to acquire qualitative data of organismic interactions but also how to obtain quantitative data of detection of specific small molecules relevant in the interaction. In addition, students are made familiar with important safety regulations and issues. The practical course (3 SWS) is included in the two week block course -Prokaryote-eukaryote-interactions” and requires regular attendance, writing a short protocol and a final exam. Qualification goals: Students are proficient in the contents of the course, understand the basic principles of key methods in microbial ecology and transfer this knowledge in an exam situation. Students have fundamental as well as up-to-date knowledge and are well trained in good biological lab practice and documentation. Specifically, the students learn how do design and critically evaluate experiments in molecular microbial ecology of organismic interactions. Students are equipped with the advanced theoretical and experimental knowledge as prerequisite to apply methods according to the topics listed above. Students having worked in small groups have improved social communication, as well as organizational skills. Discussions on the experimental design and on critical data evaluation during the course foster creative and critical thinking and the dialog with the students. Landgraf, Frank , Dr. LMU München WiSe 1415 Prof. Dr. Hartmann Anton