Praktikum Practical Course Light microscopy from bright field to multi photon microscopy

Course Content: The students will learn the theory and practical application of light microscopic techniques, starting with the basics of -classic” techniques such as bright field Koehler Illumination, phase contrast, dark field, differential interference contrast (DIC) and fluorescence microscopy. We then move on to more advanced topics such as confocal laser scanning microscopy, multi-photon microscopy and super resolution microscopy. To do so, we will mainly use the equipment of the Core Facility Bioimaging at the Biomedical Center (www.bioimaging.bmc.med.lmu.de) These techniques are applied to a variety of samples from fixed tissue sections to live cells and intravital microscopy. Small groups of students will use image processing software to generate a presentation with the images and movies recorded. Apart from giving said presentation, each student will have to pass an oral examination on one of the topics of the course. This is a 10 day course, set up with included lectures and seminar. The student has the opportunity to prepare and present a talk on one of the microscopic techniques in which case the course is counted as 6 ECTS. Otherwise it is a 3 ECTS course. The course will be in English, except all (!) participants should agree on German. Qualification goals: With this broad overview the student will be capable to determine advantages and disadvantages of various light microscopic approaches for different experimental settings and thus to select and apply the best approach for a given question. Department Biologie II LMU München SoSe 2016 Dr. Dietzel Steffen