Vorlesung Selbstorganisation von Biomolekülen

Given adequate solution conditions, biomolecular systems (e.g., proteins, lipids, DNA) are prone to self-assemble into condensed phases, such as crystals, fibrils, membranes as well as amorphous aggregates and precipitates. The propensity of biomolecules to aggregate is related to the pathogenesis of, e.g., sickle-cell anemia, type-II diabetes and Alzheimer's disease and is therefore highly relevant in medicine. We will discuss experimental techniques to characterize the structure, morphology and mechanical properties of self-assembled biomolecules as well as theoretical approaches to describe biomolecular stability against aggregation and their self-assembly pathways. To understand the formation of condensed phases of biomolecules, selected concepts from polymer physics, liquid state theory as well as colloid and surface chemistry will be introduced and applied to the study of biomolecular systems. Topics covered include (Chapters of [1] given in brackets): • The Building Blocks: Proteins, Lipids, Nucleic Acids (1) • Mesoscopic Forces (4) • Introduction to Statistical Thermodynamics (3) • Phase Transitions (5) • Aggregating Self-Assembly (8) • Surface Phenomena (9) • Biomacromolecules (10) • Polyelectrolytes (11) • Membranes (12) • Biorheology (15) • Structural Biomaterials (17) • Experimental Techniques (19) • and further advanced topics if time permits Literatur The lecture will largely follow references [1], which is online available at ULB via the university intranet or VPN, and [3].  • T.A. Waigh, The Physics of Living Processes: A Mesoscopic Approach, Wiley 2014. • J.D. Gunton, A. Shiryayev, D.L. Pagan, Protein Condensation: Kinetic Pathways to Crystallization and Disease, Cambridge University Press 2007. • K.A. Dill, S. Bromberg, Molecular Driving Forces: Statistical Thermodynamics in Biology, Chemistry, Physics, and Nanoscience, Garland 2010. • J. van der Maarel, Introduction to Biopolymer Physics, World Scientific 2007. • K.E. van Holde, W. Curtis Johnson, P. Shing Ho, Principles of Physical Biochemistry, Pearson 2006. • D. Leckband, J. Israelachvili, Intermolecular forces in biology, Q. Rev. Biophys. 34 (2001) 105. • A. Cooper, Biophysical Chemistry, RSC Publishing 2011. • P.L. Privalov, Microcalorimetry of Macromolecules, Wiley 2012. • R. Winter, F. Noll, C. Czeslik, Methoden der Biophysikalischen Chemie, Vieweg+Teubner 2011. Bemerkung In the seminar -Physics of Biomolecules- (3 CP), selected, advanced topics of the course will be discussed in detail.   On 1/12/16, 15/12/16, 12/1/17, and 19/1/17, there is a scheduling conflict with the course -Physik der Medizin- (which is obligatory for some of the participants). Therefore, our lecture -Self-Assembly of Biomolecules- will be postponed to 16:45-18:15 and hold in seminar room 25.23.02.62 on these dates. The lecture will largely follow references [1], which is online available at ULB via the university intranet or VPN, and [3].  • T.A. Waigh, The Physics of Living Processes: A Mesoscopic Approach, Wiley 2014. • J.D. Gunton, A. Shiryayev, D.L. Pagan, Protein Condensation: Kinetic Pathways to Crystallization and Disease, Cambridge University Press 2007. • K.A. Dill, S. Bromberg, Molecular Driving Forces: Statistical Thermodynamics in Biology, Chemistry, Physics, and Nanoscience, Garland 2010. • J. van der Maarel, Introduction to Biopolymer Physics, World Scientific 2007. • K.E. van Holde, W. Curtis Johnson, P. Shing Ho, Principles of Physical Biochemistry, Pearson 2006. • D. Leckband, J. Israelachvili, Intermolecular forces in biology, Q. Rev. Biophys. 34 (2001) 105. • A. Cooper, Biophysical Chemistry, RSC Publishing 2011. • P.L. Privalov, Microcalorimetry of Macromolecules, Wiley 2012. • R. Winter, F. Noll, C. Czeslik, Methoden der Biophysikalischen Chemie, Vieweg+Teubner 2011. Bemerkung In the seminar -Physics of Biomolecules- (3 CP), selected, advanced topics of the course will be discussed in detail.   On 1/12/16, 15/12/16, 12/1/17, and 19/1/17, there is a scheduling conflict with the course -Physik der Medizin- (which is obligatory for some of the participants). Therefore, our lecture -Self-Assembly of Biomolecules- will be postponed to 16:45-18:15 and hold in seminar room 25.23.02.62 on these dates. Leistungsnachweis Written examination at the end of the lecture. Medizinische Physik (BSc, PO 2012) Written examination at the end of the lecture. Universität Düsseldorf WiSe 2016/17 Dr. Platten Florian